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2 edition of Analysis of the streptomyces fertililty plasmid SCP2. found in the catalog.

Analysis of the streptomyces fertililty plasmid SCP2.

Jie Xiao

Analysis of the streptomyces fertililty plasmid SCP2.

by Jie Xiao

  • 379 Want to read
  • 21 Currently reading

Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis(Ph.D.), University of East Anglia, School of Biological Sciences, 1994.

ID Numbers
Open LibraryOL21847168M

ing. Conjugation in Streptomyces produces pocks which are zones of transient inhibition of growth of recipients. Kieser et al. () local~ ized the transfer and spread regions, involved in plasmid transfer and pock formation. respective­ ly, in plJ , a multi-copy, broad host range plasmid from Streptomyces fividallTJ). Subse­ quently. Plasmids A Desktop Resource (1st Edition) 2 | P a g e Plasmids Introduction to Addgene’s Resource Any newcomer who joins a molecular biology lab will undoubtedly be asked to design, modify, or construct a plasmid. Although the newcomer likely knows that a plasmid is a small circular piece of DNA found in bacterial cells, she mayFile Size: 2MB.

Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.? FAQ ID Yes, please follow the User-Developed Protocol ' Isolation of very low-copy plasmids from Streptomyces spp. using the QIAGEN Plasmid Maxi Kit ' (QP13). The strategy for PCR-targeting for mutagenesis of Streptomyces coelicolor. is to replace a chromosomal sequence within a. S. coelicolor. cosmid (Redenbach. et al., ) by a selectable marker that has been generated by PCR using primers with 39 nt homology extensions. The inclusion of. oriT (RK2) in the disruption cassette allowsFile Size: KB.

Four different extraction methods of small plasmid DNA from antibiotic-producing Streptomyces isolates and from the positive control S. lividans, containing the pIJ plasmid, were standardized. Among these, only one procedure allowed the detection of plasmid DNA from the positive control very effectively that was the Kieser () method. BHATTACHARYYA & SEN: GENE MANIPULATION IN STREPTOMYCES 23 al45 constructed a recombinant plasmid pNL The construction was done with a kb fragment of S. ansochromogenes, which was involved in nikkomycin biosynthesis and .


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Analysis of the streptomyces fertililty plasmid SCP2 by Jie Xiao Download PDF EPUB FB2

Gene. 35 () Eisevier GENE The Streptomyces plasmid SCP2 *: its functional analysis and development into useful cloning vectors (Restriction maps; recombinant DNA; hygromycin, thiostrepton and viomycin-resistant derivatives; stable, low-copy-number vectors; E. coli-Streptomyces shuttle vector) D.

Lydiate, F. Malpartida and D.A. Hopwood Cited by: The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Lydiate DJ, Malpartida F, Hopwood DA. Detailed restriction maps of the plasmid SCP2* and its deletion derivative pSCP were constructed.

DNA fragments carrying hygromycin (Hyg), thiostrepton (Thio) or viomycin-resistance (VioR) determinants Cited by: The plasmid SCP2, initially discovered through the occurrence of a high fertility variant, SCP2*, is a self-transmissible fertility factor capable of promoting chromosomal recombination within.

Streptomyces lividans ISP contains four small high copy number plasmids: pIJ ( kb), pIJ ( kb), pIJ ( kb) and pIJ ( kb). The three smaller species appear to be naturally occurring deletion variants of pIJ pIJ and its in vivo and in vitro derivatives were studied after transformation into S.

lividans pIJ was found to be self Cited by: Plasmid SCP2* is a 31 kb, circular, low-copy-number plasmid originally identified in Streptomyces coelicolor A3(2) as a fertility factor. The plasmid was completely sequenced.

We have constructed cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. All vectors contain the bp oriT fragment from the IncP plasmid, RK2. Transfer functions need to be supplied in trans by the E.

coli donor strain. We have incorporated into these vectors selectable antibiotic-resistance markers (Am R, Th R, Sp R) that function in Cited by: Streptomyces coelicolor A3(2) possesses two plasmids (SCP1 and SCP2) that act as sex factors. The plasmid deoxyribonucleic acid isolated from S.

coelicolor A3(2) SCP1- strains A and A had the same molecular weight and endonuclease cleavage pattern as the SCP2 plasmid. The plasmidless strain S18 SCP2- was isolated from the A X A by: 6. Documents recent research and development in streptomycetes genomics, physiology and metabolism.

An excellent source of up-to-date information. Topics include: genome architecture, conjugative genetic elements, differentiation, protein secretion, central carbon metabolic pathways, regulation of nitrogen assimilation, phosphate control of metabolism, gamma.

Lydiate DJ, Malpartida F, Hopwood DA () The Streptomyces plasmid SCP2 *, its functional analysis and development into useful cloning vectors. Gene – Google Scholar Muth G, Wohlleben W, Pühler A (a) The minimal replicon of the Streptomyces ghanaensis plasmid pSG5 identified by subcloning and Tn5 by: Streptomyces pheochromogenus Streptomyces phaeochromogenes is a bacterium species from the genus of Streptomyces.

[1] [3] [4] Streptomyces phaeochromogenes produces tyrosinate, bromoperoxidase, ditryptophenalin, phaeochromycin A, phaeochromycin B, phaeochromycin C, phaeochromycin D and phaeochromycin : Streptomycetaceae. The procedure has been used successfully for isolation of plasmids SCP2 and SCP2* as well as the plasmids listed in Table 1 (see next page) from S.

coelciolor and S. lividans strains. Genetic manipulation of Streptomyces: a laboratory manual. Hopwood. John Innes Foundation, - Genetic engineering - pages. 0 Reviews. From inside the book.

What people medium MgCl2 microcentrifuge mutations mycelium NaCl nitrocellulose nucleic acid Pasteur pipette pellet phage phenol plaques plasmid plates pocks precipitation. It makes the analysis of future reactions easier, for example, the viewing of small fragments after a restriction digest.

BioCoder version Following is the Small Scale Plasmid Isolation (Mini-Maxi prep) protocol in BioCoder, a high-level programming language for expressing biology protocols.

Abstract. The methyl-specific restriction system of Streptomyces coelicolor A3(2) was analyzed by carrying out transformations with unmethylated and methylated pSET omyces coelicolor was found to strongly restrict DNA methylated in vivo by the Dam, Dcm and Hsd modification systems of Escherichia -modified DNA was restricted as Cited by:   Plasmid pNGem3-Anti, a multicopy Streptomyces plasmid that harbours the yefMsl gene under the control of the xylanase promoter xysAp (see Methods), was used to make the expression plasmids by cloning the genes encoding the Amy α-amylase or the Xys1 xylanase under the control of the pstSp promoter (see Methods).

Plasmid Curing from Streptomyces sp: The removal of plasmid from bacterial cells lead to missing of certain characters of these cells such as antibiotic resistance or antibiotic production. In the present study the treatment of Streptomyces sp. with acridine orange (28 g /ml) result in. Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp.

We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S.

coelicolor and S. lividans but surprisingly Cited by: Journal of' General Microbiology (),Printed in Great Britain Temperature-sensitive Mutants of the Streptomyces Plasmid pIJ By ASHLEY W.

BIRCH AND JOHN CULLUM* Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute. The plasmid contains the nat1 gene from Streptomyces noursei encoding nourseothricin N-acetyl-transferase and confers resistance to the antibiotic nourseothricin of transformed yeasts.

Other plasmids conferring nourseothricin resistance: pAG35, pAG Plasmid usefull as template for PCR to create gene deletions. Plasmid SCP2-pUC from Dr. James Kadonaga's lab contains the insert SCP2 (super core promoter 2)-pUC and is published in Nat Methods. Nov. 3(11) This plasmid is available through Addgene.

Sequencing and analysis of pWTY27 The unique SacI-treated pWTY27 was cloned in an E. coli plasmid pSP72 for shotgun cloning and sequencing (see Methods). The complete nucleotide sequence of pWTY27 consisted of 14, bp with % GC content, resem-bling that of a typical Streptomyces genome (e.g.

% for S. coelicolor) [25].2 Protocol 1. Strains and plasmids for recombineering in Streptomyces coelicolor Strain/ Plasmid Relevant Genotype/Comments1 Source/ Reference Plasmids pIJ λ-RED (gam, bet, exo), cat, araC, repts Gust et al., pIJ P1-FRT-oriT-aac(3)IV-FRT-P2 Gust et al., pIJ P1-FRT-oriT-neo-FRT-P2 Gust et al., pIJ P1-FRT-neo-FRT-P2 Gust et al., File Size: KB.Practical Streptomyces Genetics Paperback – January 1, See all formats and editions Hide other formats and editions.

Price New from Used from Paperback, January 1, Format: Paperback.